Skin microbiota

Skin microbiota–host interactions [3]

The skin is a dynamic ecosystem inhabited by skin microbes (bacteria, archea, fungi and viruses) thee microbes are fundamental to skin physiology and immunity.

The skin, on the other hand, is replete in diverse and unusual lipids not found elsewhere in the body [4, 5] (Fig2 in [3]). Some of these lipids, such as sapienic acid, can have antimicrobial activities, while others, such as triglycerides, can be metabolized by microbes into free fatty acids and di- and monoglycerides that can be bioactive against other microbes or stimulatory to host cells [6, 7]. Sanford and colleagues showed that short-chain fatty acids (SCFAs) produced by microbes colonizing the skin surface lead to the inhibition of the histone deacetylase (HDAC) activity. It is known from microbes that populate the intestinal tract produce high levels of SCFAs that inhibit HDAC activity [8-10].

These findings support beneficial clinical associations between the microbial metabolome of the gut and normal immune function and provide one mechanistic explanation for how bacteria can regulate inflammation [1, 2].

Microbiome in healthy skin, update for dermatologists [11]

The skin is a complex barrier organ made of a symbiotic relationship between microbial communities and host tissue via complex signals provided by the innate and the adaptive immune systems. The skin microbiota are established during birth, the fetal skin will be colonized by microorganisms from the mother [12] . This very initial flora is low in diversity and resembles that of the delivery site, i.e. a vaginal birth will colonize a new-born with vaginal flora and a caesarean section birth with flora typical of tummy skin [13-15].

This process of skin colonization during early neonatal life is required to establish immune tolerance to commensal microorganisms [16]. During this very short time span, an abrupt inflow of highly activated regulatory T cells into neonatal skin is observed. The core skin microbiota is considered to be commensal, meaning that these microorganisms are usually harmless and most probably provide some benefit to the host. Under normal conditions these microorganism are non-pathogenic [17, 18].

From a bacteriological point of view, our skin can be considered a culture medium. Its composition is mainly the consequence of our genetics, diet, life style and the area we are living in. As a result each human skin is unique and at a genus level each microbiota present in the different areas of our skin is unique. There are four main types of environments on the human skin moist, sebaceous, dry and others. [19].  Moist areas include the axilla, inner elbow or inguinal fold. Sebaceous areas include the forehead, the alar crease (side of the nostril), the retro auricular crease (behind the ear) and the back [20] whereas the drier sites include the upper buttock area [21]. Further microenvironments include the sweat glands, the hair follicles and the dermal layers. Multiple independent detection techniques showed that bacteria are not only present on the skin surface, but are also found in deeper layers of the epidermis and even in the dermis and dermal adipose tissue [22].

These layers have specific microbiome profiles and also contain many specialized cell types such as dendritic cells, melanocytes and Langerhans cells that each express unique repertoire of functional pattern recognition receptors (PRRs) which respond actively when exposed to components of microorganisms [22-25].

Why is the skin microbiome so important?

The skin barrier and the microbiota act like a shield that protects the body against external aggressions. The composition of skin microorganism communities determines the barrier function of the skin. Altering the equilibrium in the microbiome populations might disturb the skin barrier function and activates chronic skin diseases like atopic dermatitis [26-30], psoriasis[21, 22] or acne [21, 22, 31-33].

The factors influencing the equilibrium of the microbes on the skin are summarized in the following figure.

Dreno2016

Figure 1: Factors leading to symbiosis and innate immunity response of the skin [11].

The skin microbiota modulate the expression of various innate factors, including interleukin 1a (IL-1a) [34]; components of complement [35]; and antimicrobial peptides
(AMPs), which are produced by keratinocytes and sebocytes. In the following figure the crosstalk between microbes and released metabolic substances and their impact on diverse metabolic functions and the immune system are depicted [3].

Chen2008Fig2

Figure 2: Crosstalk between skin microbiota and the host. Diverse microbes (viruses, fungi and bacteria) cover the skin surface and associated structures (hair follicles, sebaceous glands and sweat glands),
possibly forming biofilms at some sites. These microbes metabolize host proteins and lipids and produce bioactive molecules, such as free fatty acids, AMPs, phenol-soluble modulins (PSMs), cell wall components, and antibiotics158,159. These products act on other microbes to inhibit pathogen invasion, on the host epithelium to stimulate keratinocyte-derived immune mediators such as complement and IL-1, and on immune cells in the epidermis and dermis. In turn, host products and immune cell activity influence microbial composition on the skin [3].

Data from this figure show, that the non-classical MHC class I molecules, an evolutionarily ancient arm of the immune system may play an important role in promoting
homeostatic immunity to the microbiota and the skin resident bacteria can have myriad effects on the host; In addition to promoting immune barrier responses, commensal–immune interactions can also affect epithelial biology. The effects of commensal–immune interactions on many other cutaneous processes, including adnexal development, tumorigenesis, ageing, and sensory nerve function, remain to be determined.

Involvement of the peripheral nervous system may be more general and integral to skin immunity than has been previously recognized. More recently, a direct mechanistic link between neurons and immune cells has been discovered.  For instance, in the gut, mucosal neurons were found to produce a neuropeptide, neuromedin U (NMU), that binds an NMU receptor on group 2 innate lymphoid cells (ILC2s) and triggers a protective immune
response [36].

Library

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  4. Nicolaides, N., Skin lipids: their biochemical uniqueness. Science, 1974. 186(4158): p. 19-26.
  5. Strauss, J.S., P.E. Pochi, and D.T. Downing, The sebaceous glands: twenty-five years of progress. J Invest Dermatol, 1976. 67(1): p. 90-7.
  6. Drake, D.R., et al., Thematic review series: skin lipids. Antimicrobial lipids at the skin surface. J Lipid Res, 2008. 49(1): p. 4-11.
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  8. Chang, P.V., et al., The microbial metabolite butyrate regulates intestinal macrophage function via histone deacetylase inhibition. Proc Natl Acad Sci U S A, 2014. 111(6): p. 2247-52.
  9. Maslowski, K.M., et al., Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43. Nature, 2009. 461(7268): p. 1282-6.
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  13. Baviera, G., et al., Microbiota in healthy skin and in atopic eczema. Biomed Res Int, 2014. 2014: p. 436921.
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  20. Sanford, J.A. and R.L. Gallo, Functions of the skin microbiota in health and disease. Semin Immunol, 2013. 25(5): p. 370-7.
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Essentials

Essentials are naturally occurring aromatic compounds, extracted from roots, stems, blossom, bark, seeds and other parts of plants. Essentials are the effective compounds from medicinal plants and plants for consumption. In addition to their distinct odor essentials have anti bacterial and antimycotic properties. Essentials can have a strong enjoyably odor. The tempting smell is especially important for the plants, since they become dusted by bees and other insects. Thereby the odors of essentials have an important role for successful dusting.

The application of essentials on the skin works affectively against bacterial and fungal infections.  Essentials are absorbed immediately and support the regeneration and growth of healthy and resistant  skin [1]. May diseases of the human being are treated since millennia by traditionally known practice with essentials. There are reports describing, that already 4.500 years b.c. essentials were used by the Egyptians. Essentials were approved and applied for cosmetics, meditation, spiritually and used in daily life.

The application of essentials for the treatment of diseases known as “aromatherapy” is a rational therapy. The plant essentials become applied complentary with conventional medicine. There are a many reports about successful application of the aromatherapy [2]. The essentials take effect either locally when applied on the skin or are transported by the circulation to the organs treated. The essentials in the blood are not detectable already after a few minutes [3, 4]. Chemically spoken, essentials are secondary plant compounds, consisting of volatile chemical compounds. They are lipophil but do not contain fatty lipid complexes or fatty acids as do oils from plants or animals [1] .

References

1.    Mai, A., Heilen  mit  Ölen. 2015, Stuttgart: Thieme.
2.    Wabner, D. and C. Beier, Aromatherapie. 2012, Urban & Fischer.
3.    Fuchs, N., et al., Systemic absorbtion of topically applied carvone: Influence of massage technique. J. Soc. Cismet. Chem., 1997. 48(November/December): p. 277-282.
4.    Jager, W., et al., Percutaneous absorption of the montoterperne carvone: implication of stereoselective metabolism on blood levels. J Pharm Pharmacol, 2001. 53(5): p. 637-42.

Ätherische Öle

Ätherische Öle sind natürliche aromatische Verbindungen, die aus den Wurzeln, Stängeln, Blüten, Rinden, Samen und anderen Pflanzenteilen extrahiert werden. Ätherische Öle sind eine der wirksamen Bestandteile von Heil- und Genusspflanzen. Neben dem ausgeprägten Geruch besitzen ätherische Öle antibakterielle and antimykotischen Eigenschaften. Ätherische Öle können angenehm und intensiv duften. Der verführerische Duft ist für Pflanzen besonders wichtig, da sie von Bienen und Insekten bestäubt werden und die Aromen der Öle als Lockstoff für die Insekten eine wichtige Rolle bei der  Bestäubung spielen.

Beim Auftragen auf die Haut wirken ätherische Öle gegen Bakterien und Pilze. Sie werden sofort von der Haut absorbiert und stimulieren das Nachwachsen gesünderer und widerstandsfähigerer Haut [1]. Viele Erkrankungen des menschlichen Körpers werden seit Jahrtausenden mit Hilfe traditionell bekannter und überlieferter Anwendungen ätherischer Öle behandelt. Es gibt Hinweise, dass bereits 4.500 Jahre v.
Chr. die alten Ägypter ätherische Öle verwendeten. Sie waren ein wichtiger Bestandteil der ägyptischen Kultur und wurden für Kosmetik, Meditation, Heilung, Religion und in vielen anderen Bereichen des täglichen Lebens verwendet.

Die Anwendung ätherischer Öle gegen Krankheiten, bekannt als „Aromatherapie“ ist eine rationale Therapie mit pflanzlichen Ölen und Wässern, die komplementär zur konventionellen Medizin angewendet wird. Es gibt sehr viele Berichte über die erfolgreiche therapeutische Anwendung der Aromatherapie [2]. Die ätherischen Öle wirken dabei entweder lokal bei Auftragen auf der Haut oder werden über den Blutkreislauf zu den Organsystemen transportiert, wo sie ihre Wirkung entfalten. Im Blut sind sie  bereits nach wenigen Minuten nicht mehr nachweisbar [3, 4]. Chemisch gesehen sind ätherische Öle sekundäre Pflanzenstoffe, die aus flüchtigen organischen Verbindungen bestehen. Sie sind fettlöslich, haben aber keine fettigen Lipid-Komplexe oder Fettsäuren, wie sie in Pflanzenölen oder tierischem Öl vorkommen [1] .

Wirkungen ätherischer  Öle
Einige Untersuchungen sind verfügbar, die die molekularen Ursachen des positiven Effekts ätherischer Öle in Zellen untersuchen. Von den in der Onkologie angewandten ätherischen Öle und den daraus isolierten komplexen Inhaltsstoffen sind in der folgenden Tabelle ausgesuchte Untersuchungen zitiert:

Tabelle 1: Ausgewählte Beispiele veröffentlichter Studien zur molekularen Wirkung ätherischer Öle.

tabelle1

In einigen Studien wurde die Wirkung ätherischer Öle im medizinischen Umfeld benutzt. Die Studien, die sich mit der Wirkung in diesem Bereich beschäftigen, sind in Tabelle 2 zusammengestellt.

Tabelle 2: Ausgewählte Beispiele veröffentlichter klinischer Studien zur medizinischen Wirkung ätherischer Öle.

tabelle2

Literatur
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